Abstract: “Laccases are a part of the group of multicooper-containing oxidases, which in the last decades are object of intensive study due to their broad substrate specificity. Polycyclic aromatic hydrocarbons (PAHs) are a class of hazardous organic compounds which are abundant in the environment. Fluorene is a PAH, which is very toxic to aquatic organisms, causing long-term adverse effects in the aquatic environment. In the present study laccase production of Trametes versicolor was significantly enhanced by 2-methoxyphenol as inducing compound in the cultural media. Removal of fluorene was performed with crude laccase after the process of induction. The removal efficiency at the end of the process was 0.37 at 25ºC and 0.98 for the process at 37ºC. Laccase was isolated, purified and modified by using linear-dendritic-linear block copolymers. Spectral analysis show significantly more effective process of oxidation of fluorene by modified in comparison with the unmodified one.”

Keywords: Laccase, fluorene, oxidation, Trametes versicolor