Abstract: “Tetrabromobisphenol A (TBBPA) is a kind of brominated flame retardants widely used in the world. A sensitive and selective competitive indirect biotin-stretavidin amplified emzyme-linked immunosorbent assay (BA-ELISA) for the detection of TBBPA was developed. The limit of detection (IC10) and the inhibition half-maximum concentration (IC50) were 0.027ng/mL and 0.58ng/mL, respectively. Cross-reactivity values of the BA-ELISA with a set of analogues revealed a high selective. Finally, the assay was applied to the analysis of TBBPA in electronic waste samples. The results were agreed well with those of liquid chromatography method, proved that the established immunoassay were accurate and receivable. It is believed that this BA-ELISA method was suitable for rapid and sensitive screening of TBBPA in environmental monitoring.”

Keywords: flame retardant, Tetrabromobisphenol A, antigen, polyclonal antibody, immunoassay , biotin-stretavidin amplified system, electronic waste.