Abstract: “Random Amplified polymorphic DNA (RAPD) fingerprinting technique was applied to evaluate genetic similarity among nine olive (Olea europaea L.) Egyptian and no-Egyptian cultivars, (named: Hamed, Wateken, Maraki,Toffahi, Manzanillo, Picual, Dolce, Kronaki and Coratina) cultivated in experimental ranch, Faculty Agriculture Desert and Envirnmental (Fuka-Matrouh) Alexandia Universtiy, North Eastern Coast. Different polymorphism percentages were recorded for five random primers (MY1-MY2-MY3-MY4-MY5) through this investigation. Generally, all genotypes could be distinguished via five random primers under study. Fifth prime reflected high polymorphic percentage with 81 % of polymorphism. On the other hand, high similarity percentage and lowest polymorphism percentage was recorded for first primer with 27 % of polymorphism. A dendrogram was done to depict the pattern of relationships between the studied cultivars and their genetic diversity.”

Keywords: RAPD, variant identification, molecular fingerprinting, olive, Olea europaea