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Proceedings of

7th International Conference on Advances in Applied Science and Environmental Technology ASET 2017

"RV0792C; A POTENTIAL DRUG TARGET FOR MYCOBACTERIUM TUBERCULOSIS"

INOKA C. PERERA THANUSHA D. ABEYWICKRAMA
DOI
10.15224/978-1-63248-136-8-39
Pages
33 - 33
Authors
2
ISBN
978-1-63248-136- 8

Abstract: “Tuberculosis (TB), remains a pervasive global health problem by being the seventh most frequent causes of death worldwide. The GntR family of transcription factors, one of the most abundant of helix-turn-helix regulatory proteins, involves in regulating various biological processes in diverse bacteria. Out of seven putative GntR genes in M. tuberculosis genome, this study focused on the gene Rv0792c, which lie between several hypothetical proteins, a monooxygenase and an oxidoreductase. This study was designed to analyze the regulatory mechanism of Rv0792c. Gene encoding RV0792c was cloned into pET28a and subsequently the protein was purified to near homogeneity by Nickel affinity chromatography. The intergenic region (BS) between Rv0792c and monooxygenase (Rv0793) was amplified and resulting PCR product was purified by electro elution. Increasing concentration of pure protein was titrated against BS-DNA under stoichiometric conditions in an EMSA. This resulted in binding of three RV07”

Keywords: potential, drug, target, Mycobacterium, tuberculosis

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