Abstract: “Fat poses disposal problem and remain unutilized in eating establishments. We aimed at utilising Catla Catla fish fat as a cheap source of carbon for production of biosurfactant with isolated strains of bacteria. Biosurfactant is itself an eco-friendly and biodegradable chemical with multifunctional activities. We have isolated and characterized a Rhamnolipid biosurfactant produced by P. aeruginosa DSM 50071. It showed enhanced excisional wound healing activity (96%) on topical application at the wound site compared to untreated control rats (p 0.05). The higher tensile strength and higher %wound contraction for the treated group as compared to untreated revealed wound closure efficiency of the biosurfactant. Histopathological study also supported wound closure efficiency by promoting collagen synthesis. Thus our study serves dual purpose of combating environmental pollution - by solving the fish fat disposal problem with its effective utilization and reducing the cost of biosurfatant”

Keywords: Fish fat, Rhamnolipid, Tensile strength, Wound healing, Wound index