IMMOBILIZATION OF HORSERADISH PEROXIDASE ON NANOPOLYANILINE SURFACE USING SELF-ASSEMBLY TECHNIQUES FOR BIOSENSOR APPLICATIONS
Published In: 5TH INTERNATIONAL CONFERENCE ON ADVANCES IN APPLIED SCIENCE AND ENVIRONMENTAL ENGINEERING
Author(s): SITI AMIRA OTHMAN , ZUR AIN AZREEN ZAINUDDIN
Abstract: This review paper describes the immobilization of horseradish peroxidase (HRP) on nanopolyaniline (PANI) by using self-assembly technique. Some chemically synthesized conductive polymers such as PANI were used as support for HRP immobilization. Enzymes immobilized were depend on their properties of immobilize of HRP such as optimum pH, thermal stability and storage stability and all these were investigated. The structure of PANI was characterized by FTIR, FESEM and UV-VIS. The structure of PANI depends on the pH of the phosphate buffer and the type of organic solvent. Thus, the formation of monolayer especially nano-particles of PANI was prepared by using Langmuir-Blodgett technique. The contribution in the development of the biosensor application for hydrogen peroxidase (H2O2) based on the HRP modified electrode was described.
- Publication Date: 13-Mar-2016
- DOI: 10.15224/978-1-63248-086-6-50
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TRANSGENIC NATURAL KILLER CELLS
Published In: 5TH INTERNATIONAL CONFERENCE ON ADVANCES IN APPLIED SCIENCE AND ENVIRONMENTAL ENGINEERING
Author(s): ALIREZA PIRAHMADIAN , SHAHRUDIN ABDUL RAZAK
Abstract: Adaptive immunity, with its rearranging immunoglobulin and T-cell receptors, has caught most of the attention of current genetic and immunological research and overshadowed the importance of the receptors expressed by cells of the innate immune system. Natural Killer cells have evolved two main receptor systems to carry out their functions, both of them involving activating and inhibitory receptors include members from the Immunoglobulin-like superfamily as well as lectinlike receptors. Killer Immunoglobulin-like receptors (KIR) are polymorphic cell surface molecules present on Natural Killer (NK) cells which recognize classical HLA class I molecules and in doing so provide an alternative means of modulating the immune response to infected or tumoural cells. In this study we did the Separation of Natural killer cells (NK cells) from rat’s blood with the technique of LS column with the help of CD49 markers and Cultured of harvested NK cells in animal cells media (Dulbecco’s Modified Eag
- Publication Date: 13-Mar-2016
- DOI: 10.15224/978-1-63248-086-6-54
- Views: 0
- Downloads: 0