Abstract: This study was to determine the relationships between genetic variation and metal concentrations based on polluted and clean populations of Centella asiatica from the transplantation study through the use of Random Amplified Microsatellites (RAMs). The 19 primers amplified a total of 153 reproducible bands across the 8 populations of C. asiatica used for a transplantation study at week 3 and week 6. For the transplantation study under laboratory conditions, there was a similar relationship found between the genetic variations and the metal accumulations. This indicated that the roots, leaves and stems of C. asiatica were able to reflect the metals pollution of the sampling sites.

• Publication Date: 02-Jul-2017
• DOI: 10.15224/978-1-63248-125-2-01
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Abstract: In view of medicinal utilities of neem plant have been described especially for in ayurvedic remedy for alleviating of liver disorders, the present study was designed to evaluate the evidence of acute toxicity of lyophilized neem leaf powder (LNLP) and its preventive effects against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Group allocation in hepatoprotective activity study included vehicle, CCl4 (1ml/kg), Silymarin (100µg/kg/day) + CCl4 and graded doses of LNLP (50 and 100mg/ kg, BW/ day) + CCl4. On 9th day, blood was obtained for determination of biochemical parameters and liver tissue for pathological examination. There were no toxicological effects as evidenced by indications of mortality, behavior, diet consumption and tissue weights, though, some hematological parameters showed variations in their value at higher dose level. The level of protection was deliberated by using various biochemical parameters like total protein, albumin, BUN, AST, ALT and ALP levels.

• Publication Date: 02-Jul-2017
• DOI: 10.15224/978-1-63248-125-2-02
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