ELECTROCHEMICAL DNA BIOSENSOR FOR DETECTION OF PORCINE OLIGONUCLEOTIDES USING [RU(BPY)2PIP]2+ COMPLEX
Published In: 3RD INTERNATIONAL CONFERENCE ON ADVANCES IN BIO-INFORMATICS, BIO-TECHNOLOGY AND ENVIRONMENTAL ENGINEERING
Author(s): HASLINA AHMAD
Abstract: An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM
- Publication Date: 27-May-2015
- DOI: 10.15224/978-1-63248-060-6-04
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MECHANICAL PROPERTIES OF 2D CROSS-PLY E-GLASS FIBER REINFORCED THERMOPLASTIC LAMINATED COMPOSITE MATERIALS
Published In: 4TH INTERNATIONAL CONFERENCE ON ADVANCES IN CIVIL, STRUCTURAL AND MECHANICAL ENGINEERING
Author(s): IDRIS KAREN , MURAT YAZICI , CENGIZ TORCUK , HAKKI OZER , YUCEL CAN
Abstract: In accordance with the basic demand of the automotive industry, like reducing weight, lower emission values and fuel economy, researchers are working on Thermoplastic Composites (TPC) in the fields of developing, producing and recycling. The main reason of this is that Thermoplastic Composites have a lower density, higher toughness and higher impact strength besides thermoformability. Furthermore, Automotive Industry has focused attention on thermoplastic composites as a consequence of the importance of recyclable materials were risen. Cross ply [0/90]5 continuous glass fiber reinforced Polyamide (PA), Polyethylene (PE) and Polypropylene (PP) laminated composites were produced. The mechanical properties of these materials were obtained by appropriate tests.
- Publication Date: 18-Mar-2016
- DOI: 10.15224/978-1-63248-093-4-77
- Views: 0
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