Abstract: In biomedical application, it is essential to perform the detection in human serum in most of cases. However, the main difficulty of using serum is high non-specific binding between the sensor surface and serum proteins. The modifications of sensor surfaces were studied in this work. The self assemble monolayer (SAMs) and hydrophilic polymer dextran were coated on gold substrate with chemisorption method and was tested in the property of a repelling the non-specific binding of human serum by monitoring via Surface Plasmon resonance technique (SPR) gtecnique. SPR is an optical technique, which is highly sensitive on the change of the optical properties of the biomolecules in nanoscale. The results were shown that there was effect of serum dilutions on both of the sensors. It was shown the effect of increasing the human serum concentration on the SPR signal increasing. The efficiency of repelling human serum in both SAMs and dextran was shown different. Dextran gave a lower non-specific

• Publication Date: 05-May-2014
• DOI: 10.15224/978-1-63248-016-3-03
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Abstract: Pseudomonas sp. PDMZnCd2003 is a plant growthpromoting bacteria (PGPB) that was isolated from Zn/Cd contaminated soil. This research aims to study the characteristics of the Zn/Cd resistance and plant growth promoting properties of Pseudomonas sp.PDMZnCd2003 under Zn/Cd stress. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods were carried out to evaluate the lowest concentrations of Zn and/or Cd that inhibited and killed the bacterium. The MIC concentrations for Zn, Cd, Zn plus Cd and fixed Cd plus Zn were 150 mg l-1, 70 mg l-1, 60/60 mg l-1 and 20/150 mg l-1, respectively. The MBC concentrations for Cd were 300 mg l-1, while the bacterial growth recovered from treatment with 400 mg l-1 of Zn, 100/100 mg l-1 of Zn plus Cd, and 20/2000 mg l-1 of fixed Cd plus Zn. The growth tended to decrease under Zn/Cd stress, however, the plant growth properties of IAA production, N2 fixation and P solubilisation remained under Zn plus Cd at 20/20 mg l-1. Du

• Publication Date: 05-May-2014
• DOI: 10.15224/978-1-63248-016-3-04
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