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PRODUCTION OF LACCASE BY RESINICIUM BICOLOR IN SUBMERGED CULTURES: APPLICATION OF THE PLACKETT-BURMAN EXPERIMENTAL DESIGN TO SCREEN MAJOR FACTORS

Published In: INTERNATIONAL CONFERENCE ON ADVANCES IN CIVIL, STRUCTURAL, ENVIRONMENTAL AND BIO-TECHNOLOGY
Author(s): FARIDAH YUSOF , HARMEN SHAH , ZAHANGIR ALAM

Abstract: The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor ATCC 64897 under submerged condition in shake flask using Plackett-Burman design. R.bicolor potentially detoxifies ground tire rubber (GTR) and facilitates growth of Thiobacillus ferrooxidans, a bacterium that performs devulcanization of GTR but is sensitive towards rubber additives present in GTR. In this experiment out of 11 factors screened, copper sulphate and yellow flame bark powder were found to be the most significant factors affecting the laccase production. The study indicated that the screened factors can be optimized for enhanced laccase production and can potentially be utilized for attaining maximum detoxification of GTR and hence further devulcanization.

  • Publication Date: 09-Mar-2014
  • DOI: 10.15224/978-1-63248-001-9-105
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ON THE REUSE OF DREDGED MARINE SEDIMENTS: NO DUMPING, REUSE PLEASE.

Published In: INTERNATIONAL CONFERENCE ON ADVANCES IN CIVIL, STRUCTURAL, ENVIRONMENTAL AND BIO-TECHNOLOGY
Author(s): CHEE-MING CHAN

Abstract: Dredged marine sediments, conventionally considered a waste material for disposal, are either in designated offshore locations or inland containment facilities. Either of these measures incur additional costs, time and labor, not to mention the obvious lack of sustainable values. In addition, there is always the risk of transferring undesirable contaminants in the dredged materials to the disposal sites or along the transportation routes. It is however, possible to reuse this otherwise waste, with suitable and adequate pre-treatment. The material is essentially soilbased, primarily consisting sand, silt and clay with some coarse marine debris. To minimize processing time and costs, it is therefore considered most apt to harness the material’s inherent properties as a ‘soil’ and reuse it as a geomaterial in various civil engineering applications. These include reusing the sediments as a backfill material, for creating new land bases or restoring eroded ones in near-shore areas. In summa

  • Publication Date: 09-Mar-2014
  • DOI: 10.15224/978-1-63248-001-9-106
  • Views: 0
  • Downloads: 0