Abstract: We developed biosensors in which the detection element is an oligonucleotide -an aptamer- obtained through a combinatorial process. We characterized aptamers specific for different biomarkers. They can be integrated into different devices, allowing their use either in a grafted format or in solution. We developped a multiplex assay signaling the presence of nucleoside derivatives by either surface plasmon resonance or fluorescence anisotropy.

• Publication Date: 19-Apr-2015
• DOI: 10.15224/978-1-63248-043-9-80
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Abstract: The size and form of gold nanoparticles are crucial for the material properties. Particularly, anisotropic nanostructures are of particular interest because the capacity to enhance electromagnetic field upon irradiation for application in Surface Enhanced Raman Scattering spectroscopy and imaging. In the present study, biocompatible multibranched gold nanoparticles (MGNP) were synthesized by the one pot method using the piperazine moiety of HEPES buffer as reducing and stabilizing agent. The synthesis of MGNP was carried out at pH 3, 7 and 10 by using 0.2, 1.0, 2.5, 5.0 and 25.0 mM HEPES and phosphate buffer. The formation of stable MGNP in high yield was obtained using 2.5 mM HEPES buffer at pH values of 7.0 and 10.0 as evidenced by the deep blue color and the presence of red shifted resonance plasmon bands. The MGNP obtained in these conditions were used for association with cationic and anionic meso-tetrakis porphyrins, TMPyP (5, 10, 15, 20 tetrakis(1-metyl-4-pyridinio) porphyrin te

• Publication Date: 19-Apr-2015
• DOI: 10.15224/978-1-63248-043-9-102
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