RV0792C; A POTENTIAL DRUG TARGET FOR MYCOBACTERIUM TUBERCULOSIS
Published In: 7TH INTERNATIONAL CONFERENCE ON ADVANCES IN APPLIED SCIENCE AND ENVIRONMENTAL TECHNOLOGY
Author(s): INOKA C. PERERA , THANUSHA D. ABEYWICKRAMA
Abstract: Tuberculosis (TB), remains a pervasive global health problem by being the seventh most frequent causes of death worldwide. The GntR family of transcription factors, one of the most abundant of helix-turn-helix regulatory proteins, involves in regulating various biological processes in diverse bacteria. Out of seven putative GntR genes in M. tuberculosis genome, this study focused on the gene Rv0792c, which lie between several hypothetical proteins, a monooxygenase and an oxidoreductase. This study was designed to analyze the regulatory mechanism of Rv0792c. Gene encoding RV0792c was cloned into pET28a and subsequently the protein was purified to near homogeneity by Nickel affinity chromatography. The intergenic region (BS) between Rv0792c and monooxygenase (Rv0793) was amplified and resulting PCR product was purified by electro elution. Increasing concentration of pure protein was titrated against BS-DNA under stoichiometric conditions in an EMSA. This resulted in binding of three RV07
- Publication Date: 24-Sep-2017
- DOI: 10.15224/978-1-63248-136-8-39
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BIODEGRADATION OF POLYPHENOLS BY ARTHOBACTER CITREUS
Published In: 7TH INTERNATIONAL CONFERENCE ON ADVANCES IN APPLIED SCIENCE AND ENVIRONMENTAL TECHNOLOGY
Author(s): MAHESH ARVIND , NICHITH K R , SNEHA BHATT
Abstract: Polychlorophenols such as pentachlorophenols and trichlorophenols are major environmental pollutants. Tetrachlorophenol can be naturally produced while pentachlorophenol is anthropogenic in origin. The main sources of polychlorophenol contamination are from their production, application & discharge. Polychlorophenols are harmful to all life forms because they disrupt the integrity and function of biological membranes. Their metabolites are also toxic. The most efficient and economical approach for removal of polychlorophenols is bioremediation. Bacteria can degrade polychlorophenols under both aerobic and anaerobic conditions and fungi are able to aerobically metabolize them. The aerobic breakdown of aromatic compounds starts with monooxygenases or dioxygenases that introduce hydroxyl groups into the aromatic rings and further channelizes the metabolites into TCA cycle for the complete mineralization. However polychlorophenols are converted to substituted quinols before ring cleavage.
- Publication Date: 24-Sep-2017
- DOI: 10.15224/978-1-63248-136-8-43
- Views: 0
- Downloads: 0