Abstract: The objective of this study was to produce cellulolytic enzyme from paper sludge (PS), a solid waste from pulp and paper manufacturer by Geobacillus kaustophilus strain C1. When PS was used as sole carbon source, cellulolytic enzyme, mainly β-glucosidase was produced by the strain C1 which exhibited good properties, high temperature and high glucose concentration tolerance. Cellulolytic enzyme from strain C1 was afterward applied to hydrolyze ammonia-treated rice straw (ARS) for glucose production. Glucose was produced as a major hydrolysis product. Degree of saccharification of cellulose in ARS to glucose was improved when crude enzyme of strain C1 was combined with commercial Trichoderma reesei cellulase (Celluclast 1.5 L) and revealed 78.1% glucan saccharification. Glucose from ARS can be subsequently used as raw material for production of value-added products in many industries.

• Publication Date: 26-Feb-2017
• DOI: 10.15224/978-1-63248-119-1-38
• Views: 0
• Downloads: 0

Abstract: Chiral, β,γ-Unsaturated carboxylic acids derivatives are important intermediates in the synthesis of many biologically active compounds; for example anti-microtubule agents [1], Bisnorvernolepin [2] and Vineomycinone B2 [3]. Recently we have performed studies on enzymatic kinetic resolution based on esterification of carboxylic acids with orthoestres, used as a donor of alkoxy group [4,5]. As a compound for our studies, we choose 2-benzyl-2-methylbut-3-enic acid, which contains stereogenic quaternary carbon center. This compound is a substrate for the synthesis of irreversible inhibitors of a carboxypeptidase A, a representative zinc-containing proteolytic enzyme [6]. 2-Benzyl-2-methylbut-3-enic acid was synthesized in 4-step synthesis, containing enzymatic hydrolysis of benzylmethylmalonic dimethylester [6]. We propose short, two step synthesis of optically active 2-benzyl-2-methylbut-3-enic acid from commercially available tiglic acid. The results of optimization of the kinetic resol

• Publication Date: 26-Feb-2017
• DOI: 10.15224/978-1-63248-119-1-64
• Views: 0
• Downloads: 0