Abstract: Abstract- Interleukin-2 (IL-2) is a lymphokine that exerts immunoregulatory effects on a variety of cells such as T cells, activated B cells and Natural Killer (NK) cells. Its biological effects are mediated through specific interactions with cell surface receptors present on target cells. Due to its role in generating normal immune responses, IL 2 is used in the treatment of a variety of tumors and infectious diseases. The downside of IL 2 is that the molecule is rather unstable. The aim of the current research is to generate IL-2 mutant with improved potency.This objective is accomplished by the use of cloning and Site directed Mutagenesis (SDM). For this purpose,first, wild type IL-2 mRNA was isolated from cultured T lymphocytes. This was converted into cDNA with the help of Reverse Transcription-PCR. Future work includes amplification of cDNA fragments and cloning into a phagemid vector pTZ19R. Transformation will be carried out in E.coli RZ1032 and the ssDNA obtained as the end re

• Publication Date: 25-May-2014
• DOI: 10.15224/978-1-63248-028-6-04-134
• Views: 0
• Downloads: 0

Abstract: Electrical discharge machining (EDM) is one of the earliest non-traditional machining, extensively used in industry for processing of parts having unusual profiles with reasonable precision. In the present work, an attempt has been made to model Surface Roughness (SR) through Response Surface Methodology (RSM) in a die sinking EDM process. The optimization was performed in two steps using one factor at a time for preliminary evaluation and a Box-Benken design (BBD) involving three variables (Pulse on-Time, Pulse off-Time and Pulse Current) with three levels for determination of the critical experimental conditions. A copper electrode having tubular cross section was employed to machine holes of 8 mm height and 12 mm diameter on EN 353 Steel alloy workpiece. The results of analysis of variance (ANOVA) indicated that the proposed mathematical models obtained can adequately describe the performances within the limits of factors being studied. The experimental results reveal that interacti

• Publication Date: 25-May-2014
• DOI: 10.15224/978-1-63248-028-6-03-135
• Views: 0
• Downloads: 0